The data is processed by the following steps:
1) Getting
rid of low quality reads
2) Getting
rid of reads with 5' primer contaminants
3) Getting
rid of reads without 3' primer
4) Getting
rid of reads without the insert tag
5) Getting
rid of reads with poly A/G/C/T/N
6) Getting
rid of reads shorter than 16-18nt ?
7) Summarize
the length distribution of the clean reads