Assembly is the only option when working with a creature with no genome sequence,
alignment of contigs may be to ESTs, cDNAs etc
De novo Transcriptome Sequencing
zody.tiff
Filer clean reads
RNAseq reads
Functional Annotation
  - BLASTx NCBI nr
  - BLASTx Uuiprot
  - Protein domain/motif search
  - Gene Ontology
  - KEGG
  - Specific databases
Contigs
De novo assembly
zody.tiff
Remove reads which containing adaptors
 Remove reads in which unknown bases are more than 5%
 Remove low quality reads (more than half of the bases' qualities are less than 5)