A
region containing two overlapping transcripts (ACT1, from the actin gene, and
YFL040W, an uncharacterized ORF) from the Saccharomyces
cerevisiae genome is shown. Arrows point to transcription direction. The
poly(A) tags from RNA-Seq experiments are shown below these
transcripts, with arrows indicating transcription direction. The precise location of each
locus identified by poly(A) tags reveals the heterogeneity in poly(A) sites,
for example, ACT1 has two big clusters, both with a few bases of local
heterogeneity. The transcription direction revealed by poly(A) tags also helps to
resolve 3'-end overlapping transcribed regions
Poly(A) tags
from RNA-Seq
Nature
Reviews Genetics 10, 57-63
