How deep should
we go?
(a)80% of yeast genes (genome size: ~12MB) were detected at
4 million uniquely mapped RNA-Seq reads, and coverage reaches a plateau
afterwards despite the increasing sequencing depth. Expressed genes are
defined as having at least four independent reads from a 50-bp window at the
3' end.
(b)The number of unique start sites detected starts
to reach a plateau when the depth of sequencing reaches 80 million in two
mouse transcriptomes. ES, embryonic stem cells; EB, embryonic body.
Nature
Reviews Genetics 10, 57-63