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1	Genome Resequencing analysis
2	outline Download & import data Mapping reads to reference genome SNP detect DIP (InDel) detect
3	Rsequence sample data Download data from http://163.25.92.61/course/454.zip Extract the file
4	3 files are extracted from 454.zip Ecoli.FLX.fna  (Reads sequence in fasta format) Ecoli.FLX.qual  (Reads quelity in fasta format) NC_010473.gbk (E. coli str. K-12 substr. DH10B, complete genome   sequence in Genbank format)
5	How many reads in a fasta file? Extract lines with “>” character And count it
6	Import 454 reads
7	Select 2 files via  Ctrl  key
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9	Import Reference genome
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11	Mapping reads to Reference
12	Select reads
13	Select NC_010473 as reference
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15	Mapping result
16	Mapping report
17	SNP detection
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19	filter of quality
20	Annotate SNP on reference/consensus sequence
21	show SNP table
22	show genome/reads mapping
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25	DIP detection
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