What makes the differences?
Summary of second-generation sequencers
Pacific Biosciences
1 day
Single-molecule real-time DNA sequencing (SMRT)
http://www.nature.com/nrg/journal/v11/n1/images/nrg2626-f4.jpg
1000-2000
30 min
Cost: US$100
0.8 Gb
38.4 GB
zero-mode waveguides array
easier library preparation, shorter run time, longer read length, higher throughput, lower cost