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Gene List – Quality Control
•Reasonable number of genes
ranging from hundreds to thousands (e.g., 100–2,000 genes), not
extremely low or high.
•Most of the genes significantly
pass the statistical threshold for selection (e.g., selecting genes by comparing gene expression
between control and experimental cells with t-test statistics: fold changes ≥ 2 and P-values ≤0.05).
• A ‘good’ gene list should consistently
contain more enriched biology than that of a random list in the same size range during analysis in
DAVID.