Assay Consideration for Compound Library Screening (I)

Background List – How to use

•A general guideline is to set up the reference background as the pool of genes that have a chance to be selected for the studied annotation category under the scope of users’ particular study

•Default background is the entire genome-wide genes of the species matching the user’s input IDs.

•Pre-built backgrounds, such as genes in Affymetrix chips and so on, are available for the user’s choice

•In principle, a larger gene background tends to give smaller P-values.

•As most of the high throughput studies are, or at least are close to, genome-wide scope, the default background is good for regular cases in general