Assay Consideration for Compound Library Screening (I)

Benjamini

•To globally correct enrichment P-values to control family-wide false discovery rate under certain rate (e.g., ≤0.05).

•It is one of the multiple testing correction techniques (Bonferroni, Benjamini and FDR) provided by DAVID

•More terms examined, more conservative the corrections are. As a result, all the P-values get larger

•It is great if the interesting terms have significant P-values after the corrections.

•But as the multiple testing correction techniques are known as conservative approaches, it could hurt the sensitivity of discovery if overemphasizing them.