Isolate Transcript RNA
AAAAAA
AAAAAA
AAAAAA
AAAAAA
Fragment cDNA
Size Selection
Illumina Sequencing of each end
CAAA
AAAA
GGAG
CTGG
GAAA
Reverse Transcription
CAGG
*based
on Illumina approach
**strand-specific
RNA-seq protocols exist for both Illumina and SOLiD
Slide
complements of Andrew McPherson
Transcriptome
Sequencing
20,000,000
reads