Bioinformatics
In silico cloning:
In order to perform an electronic cDNA library screen, the EST sequences retrieved in this way can be used as queries in a BLASTN search of dbEST to identify over-lapping ESTs. This procedure can be reiterated with the newly identified ESTs until no additional hits are found. The ESTs isolated can be assembled into sequence contigs using computer softwares.
 emb|AJ003623|HSJ003623 H.sapiens DNA for EST MPIpl10-4B1
           Length = 556         
 Score = 46.9 bits (109), Expect = 1e-04
 Identities = 29/83 (34%), Positives = 42/83 (49%), Gaps = 8/83 (9%)
Query: 23  RLRPALCDTLITVGSQEFPAHSLVLAGVSQQLGRRGQWALGEG--------ISPSTFAQL 74
           RL+  LCD L+ VG Q+F AH  VLA  S+           E           P  F  +
Sbjct: 164 RLKGQLCDVLLIVGDQKFRAHKNVLAASSEYFQSLFTNKENESQTVFQLDFCEPDAFDNV 343
Query: 75  LNFVYGESVELQPGELRPLQEAARALGVQSL 105
           LN++Y  S+ ++   L  +QE   +LG+  L
Sbjct: 344 LNYIYSSSLFVEKSSLAAVQELGYSLGISFL 436
How to start?
TBLASTN