COMMENTS
This clone is available royalty-free through LLNL ; contact
the IMAGE Consortium (info@image.llnl.gov) for further
information.
LIBRARY
dbEST lib id: 1042
Lib Name: Soares_NFL_T_GBC_S1
Organism: Homo sapiens
Organ: pooled
Lab host: DH10B
Vector: pT7T3D-Pac
(Pharmacia) with a modified polylinker
R. Site 1: Not I
R. Site 2: Eco RI
Description: Equal amounts of plasmid DNA from three
normalized libraries
(fetal lung NbHL19W, testis NHT, and B-cell NCI_CGAP_GCB1) were
mixed, and ss circles were made in vitro. Following HAP
purification, this DNA was used as tracer in a subtractive
hybridization reaction. The driver was PCR-amplified cDNAs from
pools of 5,000 clones made from the same 3 libraries. The pools
consisted of I.M.A.G.E. clones 297480-302087, 682632-687239,
726408-728711, and 729096-731399. Subtraction by Bento Soares
and M. Fatima Bonaldo.