Find non-coding features
of interest in the sequence
Gene identification involves
4 main stages
Determine the exon-intron organization
Identify the gene
Find the putative coding
region(s)
in the sequence
motif,
signal and pattern
Blast, FASTA
Functional studies
CpG islands
Tandemly and dispersed repeats
Promoter
regions (TATA box, cap signal,
CCAAT-box)
Transcription factors, Poly-A sites
Branch point signal
CT(G,A)A(C,T)
5’ and 3’ splice sites:
AG/GUAAGU--------------PyPyPyPyPyPyPyPy-CAG/G
Open reading frame