(Linear) MAP of: gb_pr2:x03795 check: 5107 from: 1 to: 1308 LOCUS HSPDGFAR 1308 bp mRNA PRI 21-MAR-1995 DEFINITION Human mRNA for platelet derived growth factor A-chain (PDGF-A). ACCESSION X03795 VERSION X03795.1 GI:35365 KEYWORDS glycoprotein; platelet-derived growth factor 1; signal peptide. SOURCE human. . . . With 209 enzymes: * April 28, 2000 14:13 .. NciI ScrFI BslI BsiEI| AciI| HpaII| BstUI| BssKI|| HhaI| AciI CviJI|| HinP1I || BstUI HaeIII|| MnlI || HhaI EaeI ||| Cac8I | || HinP1I | EagI ||| CviJI | | || Eco57I| | Tsp509I HpaII| ||| FauI| | | || Fnu4HI|| | AciI HpyCH4V |BsrFI|| ||| MwoI || | | || TseI||| | | | | ||| ||| | || | | || |||| | TCCGCAAATATGCAGAATTACCGGCCGGGTCGCTCCTGAAGCCAGCGCGGGGAGGCAGCG 1 ---------+---------+---------+---------+---------+---------+ 60 AGGCGTTTATACGTCTTAATGGCCGGCCCAGCGAGGACTTCGGTCGCGCCCCTCCGTCGC a S A N M Q N Y R P G R S * S Q R G E A A - b P Q I C R I T G R V A P E A S A G R Q R - c R K Y A E L P A G S L L K P A R G G S A - NciI ScrFI BslI| HpaII| BssKI|| Cac8I ||| CviJI | ||| HaeIII | ||| BbvI | | ||| EaeI | | ||| Fnu4HI | | ||| AciI| | | ||| BsmFI MwoI| | | ||| AciI | Fnu4HI || | | ||| MboII | | AciI| || | | ||| BsaJI CviJI| | |AciI Fnu4HI || || | | ||| MnlI | CviJI EciI| | |FauI | || || | | ||| | | | || | | | CGGCGGCGGCCAGCACCGGGAACGCACCGAGGAAGAAGCCCAGCCCCCGCCCTCCGCCCC 61 ---------+---------+---------+---------+---------+---------+ 120 GCCGCCGCCGGTCGTGGCCCTTGCGTGGCTCCTTCTTCGGGTCGGGGGCGGGAGGCGGGG a R R R P A P G T H R G R S P A P A L R P - b G G G Q H R E R T E E E A Q P P P S A P - c A A A S T G N A P R K K P S P R P P P L - NlaIV CviJI| BglI || MwoI || BstNI | || BseRI ScrFI | || BseRI | BsaJI | | || HhaI | | BssKI | | || BstUI | | | PspGI | | || Cac8I | | | CviJI| | | || HhaI | | | HaeIII| | | || HinP1I | | | MnlI|| | | || BssHII | | | | Sau96I|| | | || HinP1I | | | | Fnu4HI||| | | || FauI| | | | | AciI|||| | | || Cac8I|| | | | | HpaII ||||| | | || BslI ||| | | | | NciI ||||| | | || HhaI ||| | | | | ScrFI ||||| | | || BstUI | ||| | | | | BslI| ||||| | | ||HinP1I | ||| | | | | FokI BssKI|| ||||| | | || MwoI| | ||| | | | | MnlI| BstF5I ||| ||||| | | ||AciI|| | ||| | | | | || | ||| ||||| | | || ||| | ||| | | | | TTCCGTCCCCACCCCCATCCCGGCGGCCCAGGAGGCTCCCCGCGCTGGCGCGCACTCCCT 121 ---------+---------+---------+---------+---------+---------+ 180 AAGGCAGGGGTGGGGGTAGGGCCGCCGGGTCCTCCGAGGGGCGCGACCGCGCGTGAGGGA a F R P H P H P G G P G G S P R W R A L P - b S V P T P I P A A Q E A P R A G A H S L - c P S P P P S R R P R R L P A L A R T P C - Cac8I BbeI MwoI| HaeII Fnu4HI || HhaI| HaeII || NlaIV|| HhaI| || SfoI|| MwoI| || BsaHI||| TseI| || HinP1I||| HinP1I || || NarI||| MnlI| || || BanI|||| Cac8I|| || || KasI|||| CviJI ||| || || MwoI ||||| MnlI ||| || || NciI ||||| BstNI | ||| || || ScrFI ||||| ScrFI | ||| || || EciI| ||||| BslI| | ||| || || BstAPI HpaII| ||||| BssKI|| | ||| || || MwoI BssKI|| ||||| PspGI|| | ||| || || BtsI |Cac8I ||| ||||| BbvI ||| | ||| || || AciI MnlI| |TspRI ||| ||||| | ||| | ||| || || | || | | ||| ||||| GTTTCTCCTCCTCCTGGCTGGCGCTGCCTGCCTCTCCGCACTCACTGCTCGCCGGGCGCC 181 ---------+---------+---------+---------+---------+---------+ 240 CAAAGAGGAGGAGGACCGACCGCGACGGACGGAGAGGCGTGAGTGACGAGCGGCCCGCGG a V S P P P G W R C L P L R T H C S P G A - b F L L L L A G A A C L S A L T A R R A P - c F S S S W L A L P A S P H S L L A G R R - Bsu36I HhaI | BstUI | | HinP1I | | MnlI | | AciI | | | Fnu4HI | | | CviJI| | | | FauI HaeIII| | | | HhaI | MnlI| | | | BstUI | | Sau96I || | | | HinP1I | | NciI| || | | | AluI BseRI| | | ScrFI| || | | | CviJI MwoI| | | BslI|| || | | | Cac8I | AciI|| | | HpaII|| || | | | AciI | | BsiHKAI ||| | | BslI||| || | | | MwoI| | | Bsp1286I ||| | | BssKI||| || | | DdeI Csp6I || | | | ||| | | |||| || | | | | GTCCGCCAGCTCCGTGCTCCCCGCGCCACCCTCCTCCGGGCCGCGCTCCCTAAGGGATGG 241 ---------+---------+---------+---------+---------+---------+ 300 CAGGCGGTCGAGGCACGAGGGGCGCGGTGGGAGGAGGCCCGGCGCGAGGGATTCCCTACC a V R Q L R A P R A T L L R A A L P K G W - b S A S S V L P A P P S S G P R S L R D G - c P P A P C S P R H P P P G R A P * G M V - BpmI FauI| BseRI || CviJI || HaeIII || Fnu4HI | || SacII | || AciI| | || BstUI| | || BsaI AciI MspA1I| | || BsmAI Fnu4HI BslI|| | || AciI | CviJI HaeII AciI||| | || BstF5I Fnu4HI | HpaII | HhaI|BsaJI||| | || RsaI FokI | | BsrFI| |HinP1I || BtgI||| | || MnlI | | | | || | | || |||| | || | TACTGATTTTCGCCGCCACAGGAGACCGGCTGGAGCGCCGCCCCGCGGCCTCGCCTCTCC 301 ---------+---------+---------+---------+---------+---------+ 360 ATGACTAAAAGCGGCGGTGTCCTCTGGCCGACCTCGCGGCGGGGCGCCGGAGCGGAGAGG a Y * F S P P Q E T G W S A A P R P R L S - b T D F R R H R R P A G A P P R G L A S P - c L I F A A T G D R L E R R P A A S P L L - BbvI AvaI| BsaJI|| HaeII|| HhaI||| HinP1I |||| BbvI MwoI| |||| BsaJI Cac8I|| |||| StyI Fnu4HI CviJI ||| |||| BsmFI| TseI| MnlI ||| |||| AvaII || BbvI || Fnu4HI Fnu4HI | ||| |||| EcoO109I || Cac8I || CviJI| TseI| | ||| |||| MnlI PpuMI || BseRI | || TseI| MnlI || | ||| |||| BstUI|Sau96I || Cac8I| | || BciVI|| Hpy188I| || | ||| |||| MnlI || HgaI| ||CviJI || | ||BsaJI ||| || || | ||| |||| | || || || | || | || | ||| TCCGAGCAGCCAGCGCCTCGGGACGCGATGAGGACCTTGGCTTGCCTGCTGCTCCTCGGC 361 ---------+---------+---------+---------+---------+---------+ 420 AGGCTCGTCGGTCGCGGAGCCCTGCGCTACTCCTGGAACCGAACGGACGACGAGGAGCCG a S E Q P A P R D A M R T L A C L L L L G - b P S S Q R L G T R * G P W L A C C S S A - c R A A S A S G R D E D L G L P A A P R L - BsaJI BslI CviJI| BstUI| HaeIII| AciI || EaeI || MnlI | || BslI | || DpnI | | || TaqI MnlI | || BstYI | | | || DpnI| PflMI | || CviJI | | | | || MboI || MnlI MnlI | | || AlwI| | | | | || MnlI || AciI | NlaIII | | || MwoI ||MboI | | | ||FauI| || | | | | | || | || | | | | || || || TGCGGATACCTCGCCCATGTTCTGGCCGAGGAAGCCGAGATCCCCCGCGAGGTGATCGAG 421 ---------+---------+---------+---------+---------+---------+ 480 ACGCCTATGGAGCGGGTACAAGACCGGCTCCTTCGGCTCTAGGGGGCGCTCCACTAGCTC a C G Y L A H V L A E E A E I P R E V I E - b A D T S P M F W P R K P R S P A R * S R - c R I P R P C S G R G S R D P P R G D R E - MlyI PleI SfaNI | NlaIV| | AvaII|| | EcoO109I|| | PpuMI|| | Sau96I|| | NciI ||| | ScrFI ||| | HpaII| ||| | BssKI|| ||| | BstF5I ||| ||| | BpmI | ||| ||| | DpnI | | ||| ||| | BstYI | | | ||| ||| | Hpy188I | | | ||| ||| | MboI | | | ||| ||| | FauI| | | | ||| ||| | FokI || | | | ||| ||| | AlwI| || | | | ||| ||| | MlyI AciI || || | | | ||| ||| | PleI Cac8I || || | | | ||| ||| | BstNI | CviJI | || || | | | ||| ||| | ScrFI | HaeIII | || || | | | ||| ||| | BssKI | | Sau96I| | || || | | | ||| ||| | PspGI | | Cac8I|| | || || | | | ||| ||| | BsmFI| | | CviJI ||| | || || | | | ||| ||| | MnlI| | | HphI ||| | || || | | | ||| ||| | HinfI || | | HinfI | ||| | || || | | | ||| ||| | | || | | | AGGCTGGCCCGCAGTCAGATCCACAGCATCCGGGACCTCCAGCGACTCCTGGAGATAGAC 481 ---------+---------+---------+---------+---------+---------+ 540 TCCGACCGGGCGTCAGTCTAGGTGTCGTAGGCCCTGGAGGTCGCTGAGGACCTCTATCTG a R L A R S Q I H S I R D L Q R L L E I D - b G W P A V R S T A S G T S S D S W R * T - c A G P Q S D P Q H P G P P A T P G D R L - BanII BsiHKAI Bsp1286I SacI AluI | CviJI | HinfI Ecl136II | NlaIV BpmI TfiI DdeI | | AvaII| DdeI MnlI | BplI| BseMII CviJI | | |Sau96I|NlaIII | | | || | | | | | || | | TCCGTAGGGAGTGAGGATTCTTTGGACACCAGCCTGAGAGCTCACGGGGTCCATGCCACT 541 ---------+---------+---------+---------+---------+---------+ 600 AGGCATCCCTCACTCCTAAGAAACCTGTGGTCGGACTCTCGAGTGCCCCAGGTACGGTGA a S V G S E D S L D T S L R A H G V H A T - b P * G V R I L W T P A * E L T G S M P L - c R R E * G F F G H Q P E S S R G P C H * - MwoI NlaIV | CviJI| | HaeIII| | BseSI Sau96I|| | Bsp1286I Fnu4HI||| | AvaI| AciI|||| | BslI MboII AluI NlaIII || BslI||||| | Hpy188I BsmFI | CviJI NspI ||MwoI |||||| | MnlI EarI| MnlI|TaqI|SfaNI | | || | |||||| | | || || || | | AAGCATGTGCCCGAGAAGCGGCCCCTGCCCATTCGGAGGAAGAGAAGCATCGAGGAAGCT 601 ---------+---------+---------+---------+---------+---------+ 660 TTCGTACACGGGCTCTTCGCCGGGGACGGGTAAGCCTCCTTCTCTTCGTAGCTCCTTCGA a K H V P E K R P L P I R R K R S I E E A - b S M C P R S G P C P F G G R E A S R K L - c A C A R E A A P A H S E E E K H R G S C - EciI HincII MlyI BstNI HinfI AccI| ScrFI Hpy188I| PleI| MspA1I BssKI | BsaJI || TaqI| AciI |HpyCH4V | | HinfI | || SalI|| MwoI| | FauI |PspGI |Bst4CI TfiI | || MnlI ||| || | | | | | | | | || | ||| GTCCCCGCTGTCTGCAAGACCAGGACGGTCATTTACGAGATTCCTCGGAGTCAGGTCGAC 661 ---------+---------+---------+---------+---------+---------+ 720 CAGGGGCGACAGACGTTCTGGTCCTGCCAGTAAATGCTCTAAGGAGCCTCAGTCCAGCTG a V P A V C K T R T V I Y E I P R S Q V D - b S P L S A R P G R S F T R F L G V R S T - c P R C L Q D Q D G H L R D S S E S G R P - Fnu4HI BstAPI| CviJI| MwoI| TseI| HpaII || MwoI || BsrFI| || NlaIV HphI|| || AciI CviJI| HpyCH4V||| || TaiI| HaeIII| BbvI Fnu4HI |||| || BtrI || Sau96I|| BsgI| TseI| |||| || MaeII| || DpnI ||| BslI| BbvI|| |||| || AhdI|| || MboI | ||| MnlI || BbvI ||| |||| || ||| || | | ||| | || | ||| |||| || CCCACGTCCGCCAACTTCCTGATCTGGCCCCCGTGCGTGGAGGTGAAACGCTGCACCGGC 721 ---------+---------+---------+---------+---------+---------+ 780 GGGTGCAGGCGGTTGAAGGACTAGACCGGGGGCACGCACCTCCACTTTGCGACGTGGCCG a P T S A N F L I W P P C V E V K R C T G - b P R P P T S * S G P R A W R * N A A P A - c H V R Q L P D L A P V R G G E T L H R L - MwoI BssSI | FauI HpyCH4V | | CviJI HgaI| Fnu4HI Fnu4HI | | | BtsI Cac8I | BstUI || TseI| TseI| | | | TspRI HgaI| | AciI |MnlI| AciI || || | | | | || | | | || | || TGCTGCAACACGAGCAGTGTCAAGTGCCAGCCCTCCCGCGTCCACCACCGCAGCGTCAAG 781 ---------+---------+---------+---------+---------+---------+ 840 ACGACGTTGTGCTCGTCACAGTTCACGGTCGGGAGGGCGCAGGTGGTGGCGTCGCAGTTC a C C N T S S V K C Q P S R V H H R S V K - b A A T R A V S S A S P P A S T T A A S R - c L Q H E Q C Q V P A L P R P P P Q R Q G - BsaJI StyI CviJI| BstNI HaeIII| MseI ScrFI MscI| MboII| BssKI | BbvI || TaiI Tsp509I || MnlI | EaeI || MaeII | CviJI | || PspGI | MnlI | || | | | | || | | | GTGGCCAAGGTGGAATACGTCAGGAAGAAGCCAAAATTAAAAGAAGTCCAGGTGAGGTTA 841 ---------+---------+---------+---------+---------+---------+ 900 CACCGGTTCCACCTTATGCAGTCCTTCTTCGGTTTTAATTTTCTTCAGGTCCACTCCAAT a V A K V E Y V R K K P K L K E V Q V R L - b W P R W N T S G R S Q N * K K S R * G * - c G Q G G I R Q E E A K I K R S P G E V R - BstUI HpaII HhaI BsaWI| HinP1I | BslI| Cac8I | | BspEI| HhaI | | | HinfI || HinP1I | | | | TfiI || MnlI HphI BseRI| | | | | CviJI | || EarI| | || | | | | | | || || GAGGAGCATTTGGAGTGCGCCTGCGCGACCACAAGCCTGAATCCGGATTATCGGGAAGAG 901 ---------+---------+---------+---------+---------+---------+ 960 CTCCTCGTAAACCTCACGCGGACGCGCTGGTGTTCGGACTTAGGCCTAATAGCCCTTCTC a E E H L E C A C A T T S L N P D Y R E E - b R S I W S A P A R P Q A * I R I I G K R - c G A F G V R L R D H K P E S G L S G R G - BfaI AvrII| BsaJI| StyI| CviJI|| HaeIII|| MlyI MboII StuI||HinfI PleI| MseI | ||| | || | GACACGGGAAGGCCTAGGGAGTCAGGTAAAAAACGGAAAAGAAAAAGGTTAAAACCCACC 961 ---------+---------+---------+---------+---------+---------+ 1020 CTGTGCCCTTCCGGATCCCTCAGTCCATTTTTTGCCTTTTCTTTTTCCAATTTTGGGTGG a D T G R P R E S G K K R K R K R L K P T - b T R E G L G S Q V K N G K E K G * N P P - c H G K A * G V R * K T E K K K V K T H L - FokI CviJI| BbvI Fnu4HI || BslI| TseI| || BstNI| AciI || || ScrFI| Fnu4HI || || BsaJI|| CviJI MnlI HphI || || BslI|| BsrGI Fnu4HI | BbvI | BstF5I| || ||BssKI||| TatI TseI| | MnlI | | CviJI| || ||PspGI||| NlaIII | || | | | | || || || |||| | | TAAAGCAGCCAACCAGATGTGAGGTGAGGATGAGCCGCAGCCCTTTCCTGGGACATGGAT 1021 ---------+---------+---------+---------+---------+---------+ 1080 ATTTCGTCGGTTGGTCTACACTCCACTCCTACTCGGCGTCGGGAAAGGACCCTGTACCTA a * S S Q P D V R * G * A A A L S W D M D - b K A A N Q M * G E D E P Q P F P G T W M - c K Q P T R C E V R M S R S P F L G H G C - MaeIII FokI| NlaIII || BsmFI | || BseRI BstF5I| | || Bst4CI AciI | RsaI|| | || RsaI | TspRI | Csp6I||| | || Csp6I| | BsrI BslI| | |||| | || || | | || | GTACATGGCGTGTTACATTCCTGAACCTACTATGTACGGTGCTTTATTGCCAGTGTGCGG 1081 ---------+---------+---------+---------+---------+---------+ 1140 CATGTACCGCACAATGTAAGGACTTGGATGATACATGCCACGAAATAACGGTCACACGCC a V H G V L H S * T Y Y V R C F I A S V R - b Y M A C Y I P E P T M Y G A L L P V C G - c T W R V T F L N L L C T V L Y C Q C A V - Hpy188I Bst4CI | ApaLI MnlI | | AvaI BsmAI Bst4CI| | | | | | || TCTTTGTTCTCCTCCGTGAAAAACTGTGTCCGAGAACACTCGGGAGAACAAAGAGACAGT 1141 ---------+---------+---------+---------+---------+---------+ 1200 AGAAACAAGAGGAGGCACTTTTTGACACAGGCTCTTGTGAGCCCTCTTGTTTCTCTGTCA a S L F S S V K N C V R E H S G E Q R D S - b L C S P P * K T V S E N T R E N K E T V - c F V L L R E K L C P R T L G R T K R Q C - BseSI BsiHKAI Bsp1286I MaeIII TspRI Tsp45I HindIII HpyCH4V | MseI | DraIII HphI | | | | | | | | GCACATTTGTTTAATGTGACATCAAAGCAAGTATTGTAGCACTCGGTGAAGCAGTAAGAA 1201 ---------+---------+---------+---------+---------+---------+ 1260 CGTGTAAACAAATTACACTGTAGTTTCGTTCATAACATCGTGAGCCACTTCGTCATTCTT a A H L F N V T S K Q V L * H S V K Q * E - b H I C L M * H Q S K Y C S T R * S S K K - c T F V * C D I K A S I V A L G E A V R S - ApoI AluI EcoRI CviJI Tsp509I | | GCTTCCTTGTCAAAAAGAGAGAGAGAGAAAAGAAAAAAAAAGGAATTC 1261 ---------+---------+---------+---------+-------- 1308 CGAAGGAACAGTTTTTCTCTCTCTCTCTTTTCTTTTTTTTTCCTTAAG a A S L S K R E R E K R K K K E F - b L P C Q K E R E R K E K K R N - c F L V K K R E R E K K K K G I - Enzymes that do cut: AccI AciI AhdI AluI AlwI ApaLI ApoI AvaI AvaII AvrII BanI BanII BbeI BbvI BciVI BfaI BglI BplI BpmI BsaI BsaHI BsaJI BsaWI BseMII BseRI BseSI BsgI BsiEI BsiHKAI BslI BsmAI BsmFI Bsp1286I BspEI BsrI BsrFI BsrGI BssHII BssKI BssSI BstAPI Bst4CI BstF5I BstNI BstUI BstYI Bsu36I BtgI BtrI BtsI Cac8I Csp6I CviJI DdeI DpnI DraIII EaeI EagI EarI EciI Ecl136II Eco57I EcoO109I EcoRI FauI Fnu4HI FokI HaeII HaeIII HgaI HhaI HinP1I HincII HindIII HinfI HpaII HphI Hpy188I HpyCH4V KasI MaeII MaeIII MboI MboII MlyI MnlI MscI MseI MspA1I MwoI NarI NciI NlaIII NlaIV NspI PflMI PleI PpuMI PspGI RsaI SacI SacII SalI Sau96I ScrFI SfaNI SfoI StuI StyI TaiI TaqI TatI TfiI TseI Tsp45I Tsp509I TspRI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AlwNI ApaI AscI AseI BaeI BamHI BbsI BbvCI BcgI BclI BglII BlpI BmrI Bpu10I BsaAI BsaBI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrDI BstBI BstEII BstXI BstZ17I ClaI DraI DrdI EcoNI EcoRV FseI FspI HpaI Hpy99I KpnI MfeI MluI MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI Ppu10I PshAI PsiI PspOMI PstI PvuI PvuII RsrII SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI