(Linear) MAPSORT of: x03795.gb_pr2 Check: 5107 from: 1 to: 1308 LOCUS HSPDGFAR 1308 bp mRNA PRI 21-MAR-1995 DEFINITION Human mRNA for platelet derived growth factor A-chain (PDGF-A). ACCESSION X03795 VERSION X03795.1 GI:35365 KEYWORDS glycoprotein; platelet-derived growth factor 1; signal peptide. SOURCE human. . . . With 209 enzymes: * April 28, 2000 15:07 .. ApaLI G'TGCA_C Cuts at: 0 1199 1308 Size: 1199 109 ApoI r'AATT_y Cuts at: 0 1303 1308 Size: 1303 5 BanI G'GyrC_C Cuts at: 0 235 1308 Size: 235 1073 BbeI G_GCGC'C Cuts at: 0 239 1308 Size: 239 1069 BglI GCCn_nnn'nGGC Cuts at: 0 152 1308 Size: 152 1156 BsaI GGTCTCn'nnnn_ Cuts at: 0 316 1308 Size: 316 992 BsaHI Gr'CG_yC Cuts at: 0 236 1308 Size: 236 1072 BsiEI CG_ry'CG Cuts at: 0 25 1308 Size: 25 1283 BsmAI GTCTCn'nnnn_ Cuts at: 0 316 1187 1308 Size: 316 871 121 Fragments arranged by size: 871 316 121 BsrI ACTG_Gn' Cuts at: 0 1130 1308 Size: 1130 178 BsrGI T'GTAC_A Cuts at: 0 1080 1308 Size: 1080 228 BssHII G'CGCG_C Cuts at: 0 168 1308 Size: 168 1140 Bsu36I CC'TnA_GG Cuts at: 0 290 1308 Size: 290 1018 BtgI C'CryG_G Cuts at: 0 343 1308 Size: 343 965 Csp6I G'TA_C Cuts at: 0 300 1081 1114 1308 Size: 300 781 33 194 Fragments arranged by size: 781 300 194 33 DraIII CAC_nnn'GTG Cuts at: 0 1245 1308 Size: 1245 63 EagI C'GGCC_G Cuts at: 0 22 1308 Size: 22 1286 Eco57I CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 57 1308 Size: 57 1251 EcoRI G'AATT_C Cuts at: 0 1303 1308 Size: 1303 5 HaeII r_GCGC'y Cuts at: 0 204 239 338 376 1308 Size: 204 35 99 38 932 Fragments arranged by size: 932 204 99 38 35 HindIII A'AGCT_T Cuts at: 0 1259 1308 Size: 1259 49 KasI G'GCGC_C Cuts at: 0 235 1308 Size: 235 1073 MaeIII 'GTnAC_ Cuts at: 0 1091 1215 1308 Size: 1091 124 93 Fragments arranged by size: 1091 124 93 NarI GG'CG_CC Cuts at: 0 236 1308 Size: 236 1072 RsaI GT'AC Cuts at: 0 301 1082 1115 1308 Size: 301 781 33 193 Fragments arranged by size: 781 301 193 33 SacII CC_GC'GG Cuts at: 0 346 1308 Size: 346 962 SfoI GGC'GCC Cuts at: 0 237 1308 Size: 237 1071 TatI w'GTAC_w Cuts at: 0 1080 1308 Size: 1080 228 Tsp45I 'GTsAC_ Cuts at: 0 1215 1308 Size: 1215 93 Enzymes that do cut and were not excluded: ApaLI ApoI BanI BbeI BglI BsaI BsaHI BsiEI BsmAI BsrI BsrGI BssHII Bsu36I BtgI Csp6I DraIII EagI Eco57I EcoRI HaeII HindIII KasI MaeIII NarI RsaI SacII SfoI TatI Tsp45I Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AlwNI ApaI AscI AseI BaeI BamHI BbsI BbvCI BcgI BclI BglII BlpI BmrI Bpu10I BsaAI BsaBI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrDI BstBI BstEII BstXI BstZ17I ClaI DraI DrdI EcoNI EcoRV FseI FspI HpaI Hpy99I KpnI MfeI MluI MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI Ppu10I PshAI PsiI PspOMI PstI PvuI PvuII RsrII SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes excluded; MinCuts: 1 MaxCuts: 350000 Excluded ranges: 388,1020 AccI AciI AhdI AluI AlwI AvaI AvaII AvrII BanII BbvI BciVI BfaI BplI BpmI BsaJI BsaWI BseMII BseRI BseSI BsgI BsiHKAI BslI BsmFI Bsp1286I BspEI BsrFI BssKI BssSI BstAPI Bst4CI BstF5I BstNI BstUI BstYI BtrI BtsI Cac8I CviJI DdeI DpnI EaeI EarI EciI Ecl136II EcoO109I FauI Fnu4HI FokI HaeIII HgaI HhaI HinP1I HincII HinfI HpaII HphI Hpy188I HpyCH4V MaeII MboI MboII MlyI MnlI MscI MseI MspA1I MwoI NciI NlaIII NlaIV NspI PflMI PleI PpuMI PspGI SacI SalI Sau96I ScrFI SfaNI StuI StyI TaiI TaqI TfiI TseI Tsp509I TspRI