!!NA_SEQUENCE 1.0
LOCUS       HSPDGFAR     1308 bp    mRNA            PRI       21-MAR-1995
DEFINITION  Human mRNA for platelet derived growth factor A-chain (PDGF-A).
ACCESSION   X03795
VERSION     X03795.1  GI:35365
KEYWORDS    glycoprotein; platelet-derived growth factor 1; signal peptide.
SOURCE      human.
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Vertebrata; Mammalia; Eutheria;
            Primates; Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Betsholtz,C., Johnsson,A., Heldin,C.H., Westermark,B., Lind,P.,
            Urdea,M.S., Eddy,R., Shows,T.B., Philpott,K., Mellor,A.L.,
            Knott,T.J. and Scott,J.
  TITLE     cDNA sequence and chromosomal localization of human
            platelet-derived growth factor A-chain and its expression in tumour
            cell lines
  JOURNAL   Nature 320 (6064), 695-699 (1986)
  MEDLINE   86203630
FEATURES             Location/Qualifiers
     source          1. .1308
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
     sig_peptide     388. .447
                     /product="signal peptide (aa -20 to -1)"
     CDS             388. .1023
                     /note="prepro PDGF-A (aa -20 to 191)"
                     /codon_start=1
                     /protein_id="CAA27421.1"
                     /db_xref="GI:35366"
                     /db_xref="SWISS-PROT:P04085"
                     /translation="MRTLACLLLLGCGYLAHVLAEEAEIPREVIERLARSQIHSIRDL
                     QRLLEIDSVGSEDSLDTSLRAHGVHATKHVPEKRPLPIRRKRSIEEAVPAVCKTRTVI
                     YEIPRSQVDPTSANFLIWPPCVEVKRCTGCCNTSSVKCQPSRVHHRSVKVAKVEYVRK
                     KPKLKEVQVRLEEHLECACATTSLNPDYREEDTGRPRESGKKRKRKRLKPT"
     misc_feature    448. .1020
                     /note="pro PDGF-A (aa 1-191)"
     misc_feature    448. .645
                     /note="propeptide (aa 1-66)"
     mat_peptide     646. .1020
                     /product="mature PDGF-A (aa 67-191)"
     misc_feature    787. .795
                     /note="pot. N-linked glycosylation site"
     polyA_site      1308
                     /note="polyA signal"
BASE COUNT      294 a    410 c    380 g    224 t
ORIGIN

  X03795  Length: 1308  April 28, 2000 14:07  Type: N  Check: 5107  ..

       1  TCCGCAAATA TGCAGAATTA CCGGCCGGGT CGCTCCTGAA GCCAGCGCGG 

      51  GGAGGCAGCG CGGCGGCGGC CAGCACCGGG AACGCACCGA GGAAGAAGCC 

     101  CAGCCCCCGC CCTCCGCCCC TTCCGTCCCC ACCCCCATCC CGGCGGCCCA 

     151  GGAGGCTCCC CGCGCTGGCG CGCACTCCCT GTTTCTCCTC CTCCTGGCTG 

     201  GCGCTGCCTG CCTCTCCGCA CTCACTGCTC GCCGGGCGCC GTCCGCCAGC 

     251  TCCGTGCTCC CCGCGCCACC CTCCTCCGGG CCGCGCTCCC TAAGGGATGG 

     301  TACTGATTTT CGCCGCCACA GGAGACCGGC TGGAGCGCCG CCCCGCGGCC 

     351  TCGCCTCTCC TCCGAGCAGC CAGCGCCTCG GGACGCGATG AGGACCTTGG 

     401  CTTGCCTGCT GCTCCTCGGC TGCGGATACC TCGCCCATGT TCTGGCCGAG 

     451  GAAGCCGAGA TCCCCCGCGA GGTGATCGAG AGGCTGGCCC GCAGTCAGAT 

     501  CCACAGCATC CGGGACCTCC AGCGACTCCT GGAGATAGAC TCCGTAGGGA 

     551  GTGAGGATTC TTTGGACACC AGCCTGAGAG CTCACGGGGT CCATGCCACT 

     601  AAGCATGTGC CCGAGAAGCG GCCCCTGCCC ATTCGGAGGA AGAGAAGCAT 

     651  CGAGGAAGCT GTCCCCGCTG TCTGCAAGAC CAGGACGGTC ATTTACGAGA 

     701  TTCCTCGGAG TCAGGTCGAC CCCACGTCCG CCAACTTCCT GATCTGGCCC 

     751  CCGTGCGTGG AGGTGAAACG CTGCACCGGC TGCTGCAACA CGAGCAGTGT 

     801  CAAGTGCCAG CCCTCCCGCG TCCACCACCG CAGCGTCAAG GTGGCCAAGG 

     851  TGGAATACGT CAGGAAGAAG CCAAAATTAA AAGAAGTCCA GGTGAGGTTA 

     901  GAGGAGCATT TGGAGTGCGC CTGCGCGACC ACAAGCCTGA ATCCGGATTA 

     951  TCGGGAAGAG GACACGGGAA GGCCTAGGGA GTCAGGTAAA AAACGGAAAA 

    1001  GAAAAAGGTT AAAACCCACC TAAAGCAGCC AACCAGATGT GAGGTGAGGA 

    1051  TGAGCCGCAG CCCTTTCCTG GGACATGGAT GTACATGGCG TGTTACATTC 

    1101  CTGAACCTAC TATGTACGGT GCTTTATTGC CAGTGTGCGG TCTTTGTTCT 

    1151  CCTCCGTGAA AAACTGTGTC CGAGAACACT CGGGAGAACA AAGAGACAGT 

    1201  GCACATTTGT TTAATGTGAC ATCAAAGCAA GTATTGTAGC ACTCGGTGAA 

    1251  GCAGTAAGAA GCTTCCTTGT CAAAAAGAGA GAGAGAGAAA AGAAAAAAAA 

    1301  AGGAATTC