(Linear) MAP of: x03795.gb_pr2 check: 5107 from: 1 to: 1308 LOCUS HSPDGFAR 1308 bp mRNA PRI 21-MAR-1995 DEFINITION Human mRNA fop platelet derived growth factor A-chain (PDGF-A). ACCESSION X03795 VERSION X03795.1 GI:35365 KEYWORDS glycoprotein; platelet-derived growth factor 1; signal peptide. SOURCE human. . . . With 209 enzymes: * MinCuts: 2 MaxCuts: 2 April 28, 2000 15:00 .. TCCGCAAATATGCAGAATTACCGGCCGGGTCGCTCCTGAAGCCAGCGCGGGGAGGCAGCG 1 ---------+---------+---------+---------+---------+---------+ 60 AGGCGTTTATACGTCTTAATGGCCGGCCCAGCGAGGACTTCGGTCGCGCCCCTCCGTCGC CGGCGGCGGCCAGCACCGGGAACGCACCGAGGAAGAAGCCCAGCCCCCGCCCTCCGCCCC 61 ---------+---------+---------+---------+---------+---------+ 120 GCCGCCGCCGGTCGTGGCCCTTGCGTGGCTCCTTCTTCGGGTCGGGGGCGGGAGGCGGGG TTCCGTCCCCACCCCCATCCCGGCGGCCCAGGAGGCTCCCCGCGCTGGCGCGCACTCCCT 121 ---------+---------+---------+---------+---------+---------+ 180 AAGGCAGGGGTGGGGGTAGGGCCGCCGGGTCCTCCGAGGGGCGCGACCGCGCGTGAGGGA BstAPI BtsI | | | GTTTCTCCTCCTCCTGGCTGGCGCTGCCTGCCTCTCCGCACTCACTGCTCGCCGGGCGCC 181 ---------+---------+---------+---------+---------+---------+ 240 CAAAGAGGAGGAGGACCGACCGCGACGGACGGAGAGGCGTGAGTGACGAGCGGCCCGCGG GTCCGCCAGCTCCGTGCTCCCCGCGCCACCCTCCTCCGGGCCGCGCTCCCTAAGGGATGG 241 ---------+---------+---------+---------+---------+---------+ 300 CAGGCGGTCGAGGCACGAGGGGCGCGGTGGGAGGAGGCCCGGCGCGAGGGATTCCCTACC BsmAI MspA1I | | TACTGATTTTCGCCGCCACAGGAGACCGGCTGGAGCGCCGCCCCGCGGCCTCGCCTCTCC 301 ---------+---------+---------+---------+---------+---------+ 360 ATGACTAAAAGCGGCGGTGTCCTCTGGCCGACCTCGCGGCGGGGCGCCGGAGCGGAGAGG EcoO109I PpuMI | TCCGAGCAGCCAGCGCCTCGGGACGCGATGAGGACCTTGGCTTGCCTGCTGCTCCTCGGC 361 ---------+---------+---------+---------+---------+---------+ 420 AGGCTCGTCGGTCGCGGAGCCCTGCGCTACTCCTGGAACCGAACGGACGACGAGGAGCCG BstYI AlwI | | | TGCGGATACCTCGCCCATGTTCTGGCCGAGGAAGCCGAGATCCCCCGCGAGGTGATCGAG 421 ---------+---------+---------+---------+---------+---------+ 480 ACGCCTATGGAGCGGGTACAAGACCGGCTCCTTCGGCTCTAGGGGGCGCTCCACTAGCTC SfaNI BstYI EcoO109I | AlwI | PpuMI | | | | | AGGCTGGCCCGCAGTCAGATCCACAGCATCCGGGACCTCCAGCGACTCCTGGAGATAGAC 481 ---------+---------+---------+---------+---------+---------+ 540 TCCGACCGGGCGTCAGTCTAGGTGTCGTAGGCCCTGGAGGTCGCTGAGGACCTCTATCTG TCCGTAGGGAGTGAGGATTCTTTGGACACCAGCCTGAGAGCTCACGGGGTCCATGCCACT 541 ---------+---------+---------+---------+---------+---------+ 600 AGGCATCCCTCACTCCTAAGAAACCTGTGGTCGGACTCTCGAGTGCCCCAGGTACGGTGA BseSI EarI SfaNI | | | AAGCATGTGCCCGAGAAGCGGCCCCTGCCCATTCGGAGGAAGAGAAGCATCGAGGAAGCT 601 ---------+---------+---------+---------+---------+---------+ 660 TTCGTACACGGGCTCTTCGCCGGGGACGGGTAAGCCTCCTTCTCTTCGTAGCTCCTTCGA MspA1I | GTCCCCGCTGTCTGCAAGACCAGGACGGTCATTTACGAGATTCCTCGGAGTCAGGTCGAC 661 ---------+---------+---------+---------+---------+---------+ 720 CAGGGGCGACAGACGTTCTGGTCCTGCCAGTAAATGCTCTAAGGAGCCTCAGTCCAGCTG TaiI MaeII | BstAPI | | | CCCACGTCCGCCAACTTCCTGATCTGGCCCCCGTGCGTGGAGGTGAAACGCTGCACCGGC 721 ---------+---------+---------+---------+---------+---------+ 780 GGGTGCAGGCGGTTGAAGGACTAGACCGGGGGCACGCACCTCCACTTTGCGACGTGGCCG BtsI | TGCTGCAACACGAGCAGTGTCAAGTGCCAGCCCTCCCGCGTCCACCACCGCAGCGTCAAG 781 ---------+---------+---------+---------+---------+---------+ 840 ACGACGTTGTGCTCGTCACAGTTCACGGTCGGGAGGGCGCAGGTGGTGGCGTCGCAGTTC TaiI MaeII | | | GTGGCCAAGGTGGAATACGTCAGGAAGAAGCCAAAATTAAAAGAAGTCCAGGTGAGGTTA 841 ---------+---------+---------+---------+---------+---------+ 900 CACCGGTTCCACCTTATGCAGTCCTTCTTCGGTTTTAATTTTCTTCAGGTCCACTCCAAT EarI | GAGGAGCATTTGGAGTGCGCCTGCGCGACCACAAGCCTGAATCCGGATTATCGGGAAGAG 901 ---------+---------+---------+---------+---------+---------+ 960 CTCCTCGTAAACCTCACGCGGACGCGCTGGTGTTCGGACTTAGGCCTAATAGCCCTTCTC GACACGGGAAGGCCTAGGGAGTCAGGTAAAAAACGGAAAAGAAAAAGGTTAAAACCCACC 961 ---------+---------+---------+---------+---------+---------+ 1020 CTGTGCCCTTCCGGATCCCTCAGTCCATTTTTTGCCTTTTCTTTTTCCAATTTTGGGTGG TAAAGCAGCCAACCAGATGTGAGGTGAGGATGAGCCGCAGCCCTTTCCTGGGACATGGAT 1021 ---------+---------+---------+---------+---------+---------+ 1080 ATTTCGTCGGTTGGTCTACACTCCACTCCTACTCGGCGTCGGGAAAGGACCCTGTACCTA MaeIII | GTACATGGCGTGTTACATTCCTGAACCTACTATGTACGGTGCTTTATTGCCAGTGTGCGG 1081 ---------+---------+---------+---------+---------+---------+ 1140 CATGTACCGCACAATGTAAGGACTTGGATGATACATGCCACGAAATAACGGTCACACGCC BsmAI | TCTTTGTTCTCCTCCGTGAAAAACTGTGTCCGAGAACACTCGGGAGAACAAAGAGACAGT 1141 ---------+---------+---------+---------+---------+---------+ 1200 AGAAACAAGAGGAGGCACTTTTTGACACAGGCTCTTGTGAGCCCTCTTGTTTCTCTGTCA BseSI MaeIII | | GCACATTTGTTTAATGTGACATCAAAGCAAGTATTGTAGCACTCGGTGAAGCAGTAAGAA 1201 ---------+---------+---------+---------+---------+---------+ 1260 CGTGTAAACAAATTACACTGTAGTTTCGTTCATAACATCGTGAGCCACTTCGTCATTCTT GCTTCCTTGTCAAAAAGAGAGAGAGAGAAAAGAAAAAAAAAGGAATTC 1261 ---------+---------+---------+---------+-------- 1308 CGAAGGAACAGTTTTTCTCTCTCTCTCTTTTCTTTTTTTTTCCTTAAG Enzymes that do cut and were not excluded: AlwI BseSI BsmAI BstAPI BstYI BtsI EarI EcoO109I MaeII MaeIII MspA1I PpuMI SfaNI TaiI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AlwNI ApaI AscI AseI BaeI BamHI BbsI BbvCI BcgI BclI BglII BlpI BmrI Bpu10I BsaAI BsaBI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrDI BstBI BstEII BstXI BstZ17I ClaI DraI DrdI EcoNI EcoRV FseI FspI HpaI Hpy99I KpnI MfeI MluI MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI Ppu10I PshAI PsiI PspOMI PstI PvuI PvuII RsrII SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes excluded; MinCuts: 2 MaxCuts: 2 AccI AciI AhdI AluI ApaLI ApoI AvaI AvaII AvrII BanI BanII BbeI BbvI BciVI BfaI BglI BplI BpmI BsaI BsaHI BsaJI BsaWI BseMII BseRI BsgI BsiEI BsiHKAI BslI BsmFI Bsp1286I BspEI BsrI BsrFI BsrGI BssHII BssKI BssSI Bst4CI BstF5I BstNI BstUI Bsu36I BtgI BtrI Cac8I Csp6I CviJI DdeI DpnI DraIII EaeI EagI EciI Ecl136II Eco57I EcoRI FauI Fnu4HI FokI HaeII HaeIII HgaI HhaI HinP1I HincII HindIII HinfI HpaII HphI Hpy188I HpyCH4V KasI MboI MboII MlyI MnlI MscI MseI MwoI NarI NciI NlaIII NlaIV NspI PflMI PleI PspGI RsaI SacI SacII SalI Sau96I ScrFI SfoI StuI StyI TaqI TatI TfiI TseI Tsp45I Tsp509I TspRI