After assembling contigs with
GelMerge, use the contig editor, GelAssemble, to review and modify the
alignments. After choosing a contig for review, GelAssemble lets you edit the
individual sequences in that contig to resolve inconsistencies. GelAssemble
creates a consensus sequence that uses the IUB nucleotide ambiguity codes. You
can modify a sequence and change the alignment in the same way you edit text
with a text editor. Although GelMerge assembles and aligns contigs
automatically, you can assemble contigs manually using GelAssemble. For
example, you could manually assemble separate contigs that do not share
sufficient overlap for GelMerge to assemble automatically. You can also
separate fragments from a contig if you believe they should not be included.
Once you are satisfied with a contig, you can store it in the sequencing
project
GelAssemble
seq03 >
GTTCATCAGTCTTGGTGGAGAAGTTCGACAGATGCCATTGGCAGATTTCACCGATGGTTC 220
seq01 >
GTTCATCAGTCTTGGTGGAGAAGTTCGACAGATGCCATTGGCAGATTTCACCGATGGTTC 540
CONSENSUS
> GTTCATCAGTCTTGGTGGAGAAGTTCGACAGATGCCATTGGCAGATTTCACCGATGGTTC
540
.........+.........+.........+.........+.........+.........+
Screen mode
Command mode
<ctr>D
<return>
