cDNA microarray 例子 :
untreated ML1 cells
mRNA
*cDNA
reverse transcription
labelling
g-irradiated ML1 cells
mRNA
AAAAA
5‘
3‘
mRNA
AAAAA…
TT TT T…
mRNA
AAAAA…
TT TT T…
*cDNA
TT TT T…
*cDNA
oligo dT primer
reverse transcriptase
+ dNTP
+Cys 3/5 dUTP or 33P dCTP
RNase A
*
*