| cDNA microarray 例子 : |
|
|
| g-irradiated |
|
| ML1
cells |
|
|
|
| untreated |
|
| ML1 cells |
|
| 5‘ |
| 3‘ |
| mRNA |
| AAAAA |
| mRNA |
| oligo dT primer |
| reverse transcription |
| mRNA |
| TT
TT T… |
|
|
|
| reverse
transcriptase |
||
| + dNTP |
||
| +Cys 3/5 dUTP or 33P dCTP |
||
| labelling |
| *cDNA |
| TT
TT T… |
| RNase A |
| TT
TT T… |
