Primer Length Minimum - Maximum - ---------------------------------------------- PCR Product Length Minimum - Maximum - ---------------------------------------------- Maximum number of primers or PCR products in output　 (range 1 thru 2500) Primer DNA concentration (nM) (range .1 thru 500.0) - Salt concentration (mM)　 (range .1 thru 500.0) - ---------------------------------------------- Select: forward primers, only reverse primers, only primers on both strands for PCR Set maximum overlap (in base pairs) between predicted PCR products Forward strand primer extension must include position Reverse strand primer extension must include position ----------------------------------------------		Reject duplicate primer binding sites on template Specify primer 3' clamp (using IUB ambiguity codes) ----------------------------------------------- Primer % G+C Minimum　 (range 0.0 thru 100.0) Maximum ----------------------------------------------- Primer Melting Temperature (degrees Celsius) Minimum　 (range 0.0 thru 200.0) Maximum ----------------------------------------------- Maximum difference between melting temperatures of two primers in PCR (degrees Celsius) (range 0.0 thru 25.0) ----------------------------------------------- Product % G+C Minimum　 (range 0.0 thru 100.0) Maximum ----------------------------------------------- Product Melting Temperature (degrees Celsius) Minimum　 (range 0.0 thru 200.0) Maximum