Open reading frame
CpG islands
Tandemly and dispersed repeats
Promoter regions (TATA box, cap signal,
CCAAT-box)
Transcription factors, Poly-A sites
Branch point signal
CT(G,A)A(C,T)
5’ and 3’ splice sites:
AG/GUAAGU--------------PyPyPyPyPyPyPyPy-CAG/G
motif, signal and pattern
Blast, FASTA
Functional studies
Identify the gene