
CELERA GENOMICS COMPLETES SEQUENCING PHAASE OF THE GENOMIC FROM ONE HUMAN BEING
Shotgun Sequencing
The whole genome shotgun technique used by
Celera to decode Drosophila melanogaster and other genomes has several phases. The first
phase involves the
creation of libraries of the complete DNA of an organism that is shredded into
small fragments.
DNA used to make the Drosophila
melanogaster libraries was provided by the Berkeley Drosophila Genome
Project (BDGP). The second phase uses
high-speed sequencing machines from Applied Biosystems to decode these fragments. In the third phase, the sequenced
fragments are assembled into their proper
order using computers employing sophisticated mathematical algorithms developed at Celera.
After the shotgun sequencing phase is complete,
a number of gaps inevitably remain
in the sequence. These gaps are typically due to the inefficient cloning of certain fragments into bacterial vectors. These
gaps are closed using a number of different
molecular biology techniques. This fourth phase, often referred to as finishing, was done in collaboration with the
BDGP. The fifth phase is the analysis and annotation of the genome, the results of
which are available in the Celera Discovery
System™