TIGR's Human Gene Index compare
with UniGene?
The HGI assemblies (and all of
TIGR's Gene Index assemblies) are made by first
clustering the EST sequences and
then assembling these clusters into consensus
sequences, or THCs(TCs for
non-human data). EST sequences are compared and
clustered together if they meet
the following criteria:
a minimum 40 base pair match
greater than 95% similarity
in the overlap region
a maximum unmatched overhang
of 20 base pairs
These clusters are then
assembled into consensus sequences using TIGR's in-house
assembly program.
UniGene links ESTs in a cluster
if the sequences have a 50 base pair overlap in the
3' untranslated region (UTR)
with 100% identity. These clusters are not run through
the more stringent assembly
process and consensus sequences are not made. For this
reason you will often find
several TIGR THCs contained within one UniGene cluster.
THCs, "Tentative Human
Consensus" sequences, are assemblies of human ESTs.