Minimal Syntax: %
gelmerge -Default
Prompted
Parameters:
-WORdsize=7
sets word size for overlap determination
-STRIngency=0.8 sets
minimum fraction of matching words in overlap
-MINOverlap=14
sets minimum length of overlap
Local Data Files:
-MATRix1=gelmergedna.cmp assigns
the scoring matrix for contig assembly
-MATRix2=gelmergelocaldna.cmp assigns the scoring matrix for vector
recognition
Optional
Parameters:
-MINIdentity=14
sets minimum run of identical bases found at least
once in an overlap between two contigs
-MAXGap=10
sets maximum gap size for overlap determination
-GAPweight=8
sets gap creation penalty in contig assembly
-LENgthweight=2
sets gap extension penalty in contig assembly
-ARChive
creates contigs from the original gel readings
-WORKing
creates contigs from individual working
fragment (with gaps removed)
-REPortfile[=Filename] writes report of recognized vector
sequences
-EXCise
removes vector sequences from single-fragment
contigs
-VECTORSTrigency=0.8 sets minimum fraction of
matches in vector recognition
-VECTORMINIdentity=12 sets minimum run of identical
bases found at least
once in a match between vector and fragment
-VECTORMAXGap=5
sets maximum gap size in first step of vector
recognition
-VECTORGAPweight=30 sets gap creation
penalty in vector recognition
-VECTORLENgthweight=3 sets gap extension penalty in
vector recognition
-NOMERge
suppresses contig assembly
-NOMONitor
suppresses screen trace of program progress
-NOSUMmary
suppresses screen summary at the end of the
program
-BATch
submits program to the batch queue