(Linear) MAP of: gb_in1:z18853 check: 4598 from: 1 to: 1144 LOCUS CECPASMR 1144 bp mRNA INV 10-MAR-1994 DEFINITION C.elegans mRNA for capping protein alpha subunit. ACCESSION Z18853 VERSION Z18853.1 GI:6687 KEYWORDS capping protein alpha subunit. SOURCE Caenorhabditis elegans. . . . With 209 enzymes: * April 28, 2000 15:38 .. TspRI BsaHI| MaeIII || BsrDI Tsp45I || AciI | DpnI | || AvaII | | MboI | | || Sau96I | | Hpy188I | | | || HgaI | | | Hpy188I MseI | | | | || | | | | | | AGTTTGAGAATGTCGGAGATCAGTGACGCCGAAAAGGTCCGCATTGCTTCGGATTTCATT 1 ---------+---------+---------+---------+---------+---------+ 60 TCAAACTCTTACAGCCTCTAGTCACTGCGGCTTTTCCAGGCGTAACGAAGCCTAAAGTAA HpaII NciI ScrFI BssKI | HhaI | | BstUI | | | SfaNI BstF5I HinP1I | | | MnlI Tsp509I | Hpy188I | FokI | | | | | | | | | | AAACACGCGCCACCCGGCGAGTTCAACGAGGTATTCAATTCGGTTCGGATGCTTTTGGAA 61 ---------+---------+---------+---------+---------+---------+ 120 TTTGTGCGCGGTGGGCCGCTCAAGTTGCTCCATAAGTTAAGCCAAGCCTACGAAAACCTT Tsp509I Hpy188I Eco57I TaiI | DpnI | MseI | AclI | | MboII MboI | | MboII | | MaeII | | | | | | | | | | | | AATGATGATCTTCTGAAGAACAAATGTGTTAATGCTATCGCTCAATATAACGTTGGACAA 121 ---------+---------+---------+---------+---------+---------+ 180 TTACTACTAGAAGACTTCTTGTTTACACAATTACGATAGCGAGTTATATTGCAACCTGTT DpnI BclI | Hpy188I | FokI MboI | MlyI| AlwNI | | BsrI BstF5I PleI|HinfI | | | | | || | | | | TTTGTGCCAGTAAAGTTGGATGGTGTTGCCAAGCAGACTCTGATCACTCCATACAATGAT 181 ---------+---------+---------+---------+---------+---------+ 240 AAACACGGTCATTTCAACCTACCACAACGGTTCGTCTGAGACTAGTGAGGTATGTTACTA MnlI TaiI| BtrI || MaeII| || TaiI DpnI || || MaeII | XmnI MboI | || || | | | | | || || TTGGGTAATGGACGTTTCTACGATGAAGTTTCAAAGAAAAGTTTCAAATACGATCACGTC 241 ---------+---------+---------+---------+---------+---------+ 300 AACCCATTACCTGCAAAGATGCTACTTCAAAGTTTCTTTTCAAAGTTTATGCTAGTGCAG DpnI BglII | BstYI | MboI | FokI| | AciI || | Fnu4HI || | CviJI| || | HaeIII| || | HgaI MboII| || | HinfI MlyI | BsaHI BslI || || | BstF5I CviJI | PleI| | BslI | | || || | | | | || | | | CGTAAAGAGGCCGCAGATCTTCAACCACATCCAGCCGAGTCTGGAATAACCGAACAATGG 301 ---------+---------+---------+---------+---------+---------+ 360 GCATTTCTCCGGCGTCTAGAAGTTGGTGTAGGTCGGCTCAGACCTTATTGGCTTGTTACC DpnI AluI EarI BclI | MboII CviJI SapI TaqI MboI | BstUI | | | | | | | CGTCAAGCTCTTCAAACTCAACTCGATATATATATTGATGATCATTACGCGAAAAGTGGA 361 ---------+---------+---------+---------+---------+---------+ 420 GCAGTTCGAGAAGTTTGAGTTGAGCTATATATATAACTACTAGTAATGCGCTTTTCACCT Tsp509I SfaNI | HinfI | | TfiI | | TaqI | | | HpyCH4V | | | | TaqI BsaBI| | | | | | || | | | | ACGGGAGTGGTTTTTGCTCGAAACGGAGTTTTCACGATTTGCATCGAATCTCATCAATTC 421 ---------+---------+---------+---------+---------+---------+ 480 TGCCCTCACCAAAAACGAGCTTTGCCTCAAAAGTGCTAAACGTAGCTTAGAGTAGTTAAG ApoI Tsp509I Hpy188I HpaII CviJI | MaeIII MboII| BsrFI| | | | || || CAGCCAAAAAATTTCTGTAACGGAAGATGGCGTTCGGAGTGGAATGTGCCGGTTGGCGAT 481 ---------+---------+---------+---------+---------+---------+ 540 GTCGGTTTTTTAAAGACATTGCCTTCTACCGCAAGCCTCACCTTACACGGCCAACCGCTA DdeI DpnI | ApoI BstYI | | Tsp509I MboI | |AlwI BseMII | | | | | | | GGTAAATCAGGATCTCAGGAGATGAAAGGAAAAATTTTGTCGCAAGTTCATTATTACGAA 541 ---------+---------+---------+---------+---------+---------+ 600 CCATTTAGTCCTAGAGTCCTCTACTTTCCTTTTTAAAACAGCGTTCAAGTAATAATGCTT BseMII HpyCH4V | TaiI| | HpaII MboII|| | BsrFI| MaeII ||| | Hpy188I CviJI| BbsI | ||| | DdeI| BsgI || MseI CviJI | | ||| | || | || | | GACGGAAACGTGCAGTTGTTCTCTGAGAAAGAGCCGGTTCTTAAAGTCAATGTTTCGGCT 601 ---------+---------+---------+---------+---------+---------+ 660 CTGCCTTTGCACGTCAACAAGAGACTCTTTCTCGGCCAAGAATTTCAGTTACAAAGCCGA BsmAI Hpy188I| BsaJI|| Tsp509I MnlI ||| TaqI AciI XmnI NlaIII | EarI|| MboII | | | | | ||| | GACTTCGACAAAACCGCCAAAGAAATAATTCATGCCATTTCCGAGGAAGAGACTATATAT 661 ---------+---------+---------+---------+---------+---------+ 720 CTGAAGCTGTTTTGGCGGTTTCTTTATTAAGTACGGTAAAGGCTCCTTCTCTGATATATA RsaI Csp6I| BsmI|| BsrGI|| Hpy188I TatI||Tsp509I Hpy188I | ||| | | CAGAATGCTGTACAAGAGAATTACGCTAATATGTCTGATACCACATTCAAAGCACTTCGT 721 ---------+---------+---------+---------+---------+---------+ 780 GTCTTACGACATGTTCTCTTAATGCGATTATACAGACTATGGTGTAAGTTTCGTGAAGCA DdeI MfeI HhaI| Tsp509I MaeIII BstUI || Hpy99I | BsrI | HinP1I || BsrI CviJI Tsp509I | | | | | || | | | CGCCAATTGCCAGTTACTCGCGCTAAGATGGACTGGAATAAGGCTCAAACCTACCGAATT 781 ---------+---------+---------+---------+---------+---------+ 840 GCGGTTAACGGTCAATGAGCGCGATTCTACCTGACCTTATTCCGAGTTTGGATGGCTTAA BbsI MboII | GGTCAGGAAATGAAGTAATCACAAAATCGTTATCACATCGCTTTGCTTCAAGTTCGTCTG 841 ---------+---------+---------+---------+---------+---------+ 900 CCAGTCCTTTACTTCATTAGTGTTTTAGCAATAGTGTAGCGAAACGAAGTTCAAGCAGAC MnlI MboII MnlI Tsp509I TaqI| | | | || CTGGTCTTCATCATCTTCCTAATCCTCTCCAAAAACCAAAAAATCCTCCAATTTCGACCT 901 ---------+---------+---------+---------+---------+---------+ 960 GACCAGAAGTAGTAGAAGGATTAGGAGAGGTTTTTGGTTTTTTAGGAGGTTAAAGCTGGA HpaII BslI| TaiI CviJI| BtrI | HaeIII| MaeII| | EaeI || BfaI || | | || | || | TTATTTCTCCCACTTTCCTTTATCCTTTGGCCGGACACTAGACGTGCTTTACAACATAAT 961 ---------+---------+---------+---------+---------+---------+ 1020 AATAAAGAGGGTGAAAGGAAATAGGAAACCGGCCTGTGATCTGCACGAAATGTTGTATTA Fnu4HI MspA1I| TseI| NlaIII BbvI AciI || BspHI |Tsp509I | | || | | | ACCCTTCGTTTGCTCCGCTGCTTCTTTTTTCATGATTTGATAATTTGTATCATCTATGTA 1021 ---------+---------+---------+---------+---------+---------+ 1080 TGGGAAGCAAACGAGGCGACGAAGAAAAAAGTACTAAACTATTAAACATAGTAGATACAT HinfI Tsp509I TfiI Tsp509I | | | TCTAATTTTATTGTATGGTATGATTCATTTTTCAATTATATATTATTTGTGTGTGTGTGA 1081 ---------+---------+---------+---------+---------+---------+ 1140 AGATTAAAATAACATACCATACTAAGTAAAAAGTTAATATATAATAAACACACACACACT MseI | TTAA 1141 ---- 1144 AATT Enzymes that do cut: AciI AclI AluI AlwI AlwNI ApoI AvaII BbsI BbvI BclI BfaI BglII BsaBI BsaHI BsaJI BseMII BsgI BslI BsmI BsmAI BspHI BsrI BsrDI BsrFI BsrGI BssKI BstF5I BstUI BstYI BtrI Csp6I CviJI DdeI DpnI EaeI EarI Eco57I Fnu4HI FokI HaeIII HgaI HhaI HinP1I HinfI HpaII Hpy99I Hpy188I HpyCH4V MaeII MaeIII MboI MboII MfeI MlyI MnlI MseI MspA1I NciI NlaIII PleI RsaI SapI Sau96I ScrFI SfaNI TaiI TaqI TatI TfiI TseI Tsp45I Tsp509I TspRI XmnI Enzymes that do not cut: AatII AccI Acc65I AfeI AflII AflIII AgeI AhdI ApaI ApaLI AscI AseI AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI BglI BlpI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaWI BseRI BseSI BsiEI BsiHKAI BsiWI BsmBI BsmFI Bsp1286I BspEI BspMI BsrBI BssHII BssSI BstAPI BstBI Bst4CI BstEII BstNI BstXI BstZ17I Bsu36I BtgI BtsI Cac8I ClaI DraI DraIII DrdI EagI EciI Ecl136II EcoNI EcoO109I EcoRI EcoRV FauI FseI FspI HaeII HincII HindIII HpaI HphI KasI KpnI MluI MscI MslI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI Ppu10I PpuMI PshAI PsiI PspGI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI Tth111I XbaI XcmI XhoI XmaI