Data Processing – Raw
Nucleotide Sequence
EST or SAGE clones sequenced
MegaBRACE 1000
Chromas
High quality
Chromas
Poor quality
Abi format
High quality
Poor quality
Fasta format
sequence
sequence
PC
UNIX
PHRED algorithm
Remove uncalled/miscalled bases & vector
sequence
Ewing B et al. (1988)
Base-calling of automated sequencer traces using phred. I. Accuracy
assessment. Genome Res. 8(3):175-85
Base-calling of automated sequencer traces using phred. II. Error
probabilities. Genome Res. 8(3):186-94