1.Download the
sequences Gene, RNA and Protein
2.Upload to SeqWEB
ANALYSIS:
1.Exon/intron
organization.
Use (1) BESTFIT & GAP (“gene”
vs “rna”)
(2) Genome Blastn
2.Opening Reading
Frame
Use MAP to find the ORF
Use TRANSLATE to write the
ORF
Compare your ORF with “protein”
3. Protein Domain Search (NCBI CD Search,
Interpro)
4. Protein Sequence Analysis
see next page