gcg2 17% ls best.pair capb_human.sw k02938.gb_ov capb.txt capb_mouse.sw pileup.figure capb_caeel.swissprot fetch.msf x15785.gb_ov capb_chick.sw gap.pair gcg2 18% pileup @capb.txt PileUp creates a multiple sequence alignment from a group of related sequences using progressive, pairwise alignments. It can also plot a tree showing the clustering relationships used to create the alignment. 1 CAPB_CHICK 277 aa 2 CAPB_MOUSE 277 aa 3 CAPB_HUMAN 272 aa 4 CAPB_CAEEL 270 aa What is the gap creation penalty (* 8 *) ? What is the gap extension penalty (* 2 *) ? This program can display the clustering relationships graphically. Do you want to: A) Plot to a FIGURE file called "pileup.figure" B) Plot graphics on HP7550 attached to /dev/tty15 C) Suppress the plot Please choose one (* A *): The minimum density for a one-page plot is 4.0 sequences/100 platen units. What density do you want (* 4.0 *) ? What should I call the output file name (* capb.msf *) ? list.msf Determining pairwise similarity scores... 1 x 2 5.14 1 x 3 4.79 1 x 4 3.63 2 x 3 4.82 2 x 4 3.62 3 x 4 3.63 Aligning... 1 .............-.. 2 .............-. .............-.. 3 .............-. .............-.. FIGURE instructions are now being written into pileup.figure. Total sequences: 4 Alignment length: 278 CPU time: 00.34 Output file:/disk2/usr/people/petang/LECTURE5/list.msf