Primer Length
Minimum -
Maximum -
----------------------------------------------PCR Product
Length
Minimum -
Maximum
-
----------------------------------------------Maximum number of primers
or PCR products in output (range 1 thru 2500)
Primer DNA concentration
(nM) (range .1 thru 500.0)
-
Salt concentration
(mM) (range .1 thru 500.0) -
----------------------------------------------Select:
forward primers, only
reverse primers, only
primers on both
strands for PCR
Set maximum overlap (in
base pairs) between predicted PCR products
Forward
strand primer extension must include
position
Reverse
strand primer extension must include
position
----------------------------------------------
Reject duplicate primer
binding sites on template
Specify primer 3' clamp
(using IUB ambiguity codes)
-----------------------------------------------
Primer % G+C
Minimum (range 0.0 thru 100.0)
Maximum
-----------------------------------------------Primer Melting
Temperature (degrees Celsius)
Minimum
(range 0.0 thru 200.0)
Maximum
-----------------------------------------------Maximum
difference between melting temperatures of two
primers in PCR
(degrees Celsius) (range
0.0 thru 25.0)
-----------------------------------------------
Product % G+C
Minimum (range 0.0 thru 100.0)
Maximum
-----------------------------------------------
Product Melting Temperature (degrees Celsius)
Minimum (range 0.0 thru 200.0)
Maximum