NCBI
Blast vs GCG Blast
Unix system
Smaller database
Data not interlinked
Built your own database
Fast
Support multiple search
Output file easier to parse
GCG Blast
WWW system
Larger database
Interlinked Data
Slow
Single search only
NCBI Blast
Download CDK2 amino acid sequence
Upload to GCG
Reformat
GCG> blast -BAT
Copy & Paste
http://www.ncbi.nlm.nih.gov/BLAST/