1.Download the sequences Gene, RNA and
Protein
2.Upload to SeqWEB
ANALYSIS:
1.Exon/intron organization.
Use (1) BESTFIT &
GAP (“gene” vs “rna”)
(2) Genome Blastn
2.Opening Reading Frame
Use MAP to find the
ORF
Use TRANSLATE to
write the ORF
Compare your ORF with
“protein”
3. Protein Domain Search
(NCBI CD Search, Interpro)
4. Protein Sequence
Analysis
see next page