▲ Relational database (Oracle 8i)
▲ Automatic data validation
▲ Quality score generation
▲ Automatic trimming of low-quality, vector, adaptor, poly-A tails,
low-complexity
and contaminant sequences
▲ Automatic running of selected blast
algorithms, with user-defined parameters, user selected reference databases,
and storage of top results (by user- defined cutoffs) in the database
▲ Includes a web interface for viewing
the data in the database, according to the permissions allowed to the viewer
(by individual, project, lab or institution)
▲ Includes a Java tool for dbEST
submission of newly generated ESTs at intervals define by the users
▲ System can be readily and simply deployed at any of the partner's
institutions
▲ Includes methods for defining a Unigene set for a library.
Additional
functionalities are needed by the members of the current co-development
group, including:
▲ Tissue or organism, integration of gene expression data.
▲ Annotations: Gene ontology
annotations, functional motif annotation, metabolic pathways annotations, signal
transduction pathways.
Basic
Features and Tools of an Automated
EST
Analysis Pipeline