Marilyn Monroe
Marilyn was printed using a MicroGrid Compact with MicroSpot 10K Quill pins onto Corning GAPS2 slides. Cy3 and Cy5 labelled M13FWD oligonucleotides were made to 1µM in 1 x EZRays spotting solution (Apogent Discoveries), 0.01% N-lauroyl sarcosine. Eight 2-fold dilutions of the oligonucleotides were made into the same spotting buffer. A 20µl aliquot of each oligonucleotide dilution were placed in the appropriate wells of Greiner 384 well v bottom microplates.
The Art of Microarray
The image of Marilyn was downloaded from the web, pixelised and grayscaled using image manipulation software. Pixel information was extracted to Excel from which grid patterns were generated. Ms Monroe was scanned at 10µm resolution using the ArrayWorX scanner from Applied Precision.