Mona Lisa
Mona was printed using a
MicroGrid Compact with MicroSpot 10K Quill pins onto Corning GAPS2 slides.
Cy3 labelled M13FWD
oligonucleotides were made to 1µM in 1 x EZRays spotting solution (Apogent
Discoveries), 0.01% N-lauroyl sarcosine. Eight
2-fold dilutions of the oligonucleotides were
made into the same spotting buffer. A 20µl aliquot of each oligonucleotide dilution
were placed in the appropriate wells of
Greiner 384 well v bottom microplates.The Art of Microarray
The image of Mona was
downloaded from the web, pixelised and grayscaled using image manipulation
software. Pixel information was extracted to Excel from which grid patterns
were generated.
The Mona Lisa was scanned
at 10µm resolution using the ArrayWorX scanner from Applied Precision.