Data Transformation
Transformed
data {(M,A)}n=1..5184:
M = log2(R/G) (ratio),
A = log2(R·G)1/2 = 1/2·log2(R·G) (intensity signal)
Û R=(22A+M)1/2, G=(22A-M)1/2
“Observed” data {(R,G)}n=1..5184:
R =
red channel signal
G = green channel signal
G = green channel signal
(background corrected or
not)