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An H&E stained slide produced from a tissue array block is depicted in this diagram. When the arrayed pattern is magnified, the individual circular portions of tissue are clearly seen.
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The whole process includes the selection and treatment of the glass substrates, visual monitoring and recording the deposition of each element printed on the array, and the final characterization and hybridization of statistically representative samples of arrays from each lot produced
We do quality control our arrays at every step of the process. The arrays that the customer receive are the ones that passed these quality control.
The combination of microarray printing know-how, technology innovation, in process QC and industrialization forms the basic for Agilent Sure Print technology.
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As a quick overview of the experimental procedures,
Firstly take two samples, these could be two cell populations, tissues disease states etc
Extract the RNA, There are several methods depending on amount of starting material, however past work has shown that this is one of the largest sources of variation in the experiment. RNA quality is very important and reliable data is reliant on good quality RNA
Next the RNA is reverse transcribed to cDNA, depending on the amount of RNA this may involve an amplification step.
The two samples are then labelled with one of two different flourophores cy5 and cy3 mixed then applied to the array and hybridised overnight
The slide is then washed and scanned, to produce two tif files, one for each of the samples and flourophore used.
The images are then overlaid and the pixel intensities for each probe quantified
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Emphasize full complexify
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