Default assumptions in phylogenetics
1.The sequence is correct and originates from the specified source.
2.The sequences are homologous (i.e., are all descended in some way from a shared ancestral sequence).
3.Each position in a sequence alignment is homologous with every other in that alignment.
4.Each of the multiple sequences included in a common analysis has a common phylogenetic history with the others (e.g., there are no mixtures of nuclear and organellar sequences).
5.The sampling of taxa is adequate to resolve the problem of interest.
6.Sequence variation among the samples is representative of the broader group of interest.
7.The sequence variability in the sample contains phylogenetic signal adequate to resolve the problem of interest.
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