1.The components. Discover all of the genes in
the genome and the subset of genes, proteins, and other small
molecules constituting the pathway of interest. If possible, define
an initial model of the molecular interactions
governing pathway function (how?).
2.
2.Pathway perturbation. Perturb each
pathway component through a series of genetic or environmental manipulations.
Detect and quantify the corresponding global cellular response
to each perturbation.