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3	改變二十世紀的大事
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6	"Differential displaying" Differential displaying Suppressive subtractive hybridization (SSH) Sequencing of expressed sequence tags (EST), serial analysis gene expression (SAGE & Long SAGE)  Hybridization to microarrays
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48	"Ink-jet printhead draws up DNA..." Ink-jet printhead draws up DNA Ink-jet printer microarrays Printhead moves to specific location on solid support DNA ejected through small hole Used to spot DNA or synthesize oligonucleotides directly on glass slide Use pioneered by Agilent Technologies, Inc.
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51	"Oligonucleotides" Oligonucleotides usually at least 20–25 bases in length, optimal with 45~60 bps long 10–20 different oligonucleotides for each gene Oligonucleotides for each gene selected by computer program to be the following: unique in genome (4 (20 to 25) =2(40 to 50) >> 3*109 = 230), not likely to appear twice non-overlapping (if the sequence length is too short then specificity is low, whereas if the length is too long, self-hybridization could happen) Composition based on design rules empirically derived rules (ratio of G-C pairs vs. A-T pairs which could affect the melting temperature of the seq., ie. Tm = 64.9+0.41*(GC%) -675/L)
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