Differential
displaying
Advantages
üto compare multiple experimental samples simultaneously
üto identify genes that
are either up or down-regulated in one sample relative to another
Several significant
limitations of the original protocol were:
Øthe requirement of large quantities of mRNA
Øa bias against the
identification of rare transcripts.
Ønot a quantitative method with a
high rate of false positives,
or
Øgene fragments that seem to be differentially expressed as an artifact