Subtractive
hybridization
Subtractive
hybridization (or subtraction) is a method for rapid isolation of differentially distributed nucleic
acids (differentially expressed, differentially present, or differentially
arranged in two or more different sources: different cells, cell populations
or cell types, different tissues, disease, or development stages). The Suppressive subtractive hybridization (SSH) method is designed to selectively amplify differentially expressed transcripts while suppressing the amplification of abundant
transcripts, thus eliminating the need to separate single- and double-stranded
molecules.
