•Resolution
–standard 10mm (100,000 atoms wide)
–100mm spot on chip = 10
pixels in diameter
•Image format
–TIFF 16 bit (64K grey levels)
–1cm x 1cm image at 16
bit = 2Mb (uncompressed)
•Separate image for each fluorescent sample
–channel 1, channel 2
Image Processing
Overlay
images
and
normalize
Image
process
and
analyze
Green
channel
Red
channel
Scan
and detect with
confocal
laser system
laser
detection
1)Laser beam excites each spot of DNA
2)Amount of fluorescence detected
3)Different lasers used for different wavelengths