ØAddressing or gridding
–locate centers, assigning coordinates to each of the spots
ØSegmentation or spot picking
–classifying pixels either as foreground or as background
ØIntensity extraction (for
each spot)
–Foreground fluorescence intensity pairs (R, G)
–Background intensities
–Quality measures
ØInformation extraction
–for each spot of the array, calculates signal intensity pairs,
background and quality measures
Raw
(combined) image
Gridded
Spots
picked & flagged
Intensity
Image Processing
signal
background