Global (LOWESS) Normalization -within
slide normalization
(R, G) « (M, A) Transformation
“Observed” data {(R, G)}n=1..n
R = red
channel signal
G =
green channel signal
(background corrected or not)
Transformed data {(M, A)}n=1..n
M = log2(R/G) (ratio),
A = log2(R × G)1/2 = 1/2 × log2(R × G)
(intensity)
Û R=(22A+M)1/2, G=(22A-M)1/2