Blast NCBI Database
Create a folder “NETBLAST” in “C” Drive
Download netblast-2.2.18-ia32-win32.exe
Decompress the file
COMMAND
開始à執行àcmd
cd/
cd\NETBLAST\
blastcl3 -p blastn -i
c:\NETBLAST\FILENAME -d nr -o
c:\NETBLAST\FILENAME-BLASTn_-nr.txt
-p program
-I input file
-d database
-o output file
OPTIONS:
-m 8 tubular output
-b 1 list one description
-v 1 show one alignment
EXERCISE:
Use the sequence
file from last week’s homework to do a netblast WITH & WITHOUT options