Blast Local Database
Create a folder “NETBLAST” in “C” Drive
Download blast-2.2.18-ia32-win32.exe
Decompress the file
COMMAND
開始à執行àcmd
cd/
cd\BLAST\bin
CREATE LOCAL
DATABSE
formatdb -p T -o T
-i c:\BLAST\FILENAME (protein)
formatdb -p F -o T
-i c:\BLAST\FILENAME
(nucleotide)
LOCAL
BLAST
blastall -p blastp -i c:\BLAST\FILENAME
-d c:\BLAST\DATABASE -o
c:\BLAST\BLASTx-FILENAME.txt
-p program
-I input file
-d database
-o output file
OPTIONS:
-m 8 tubular output
-b 1 list one description
-v 1 show one alignment
EXERCISE:
Reformat the
sequence file from last week’s homework as a local database.
Use the same
sequence to do a blast WITH & WITHOUT options