| Expressed Sequence Tags (EST) | |
| ¡@ | EST is a well-established technology for the generation of gene expression profiles and a powerful tool for genome annotation. For the interest of comparative transcriptomics, we generated more than 70,000 ESTs rom T. vaginalis, T. foetus and P. hominis. Details of The ¡§Trichomonadidae Expression Sequence Tag (EST) Project¡¨can be find on the TvXpress website. |
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| ¡@ | TvXpress Database |
| ¡@ | Wikipedia on Expressed Sequence Tag |
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| Next-generation Sequencing (NGS) | |
| ¡@ | Next-Generation Sequencing (NGS) technologies have found broad applicability in functional genomics research. Their applications in the field have included gene expression profiling, genome annotation, small ncRNA discovery and profiling, and detection of aberrant transcription, which are areas that have been previously dominated by microarrays. NGS platforms could easily generate a throughput of more than 1000 mega base pairs per run, which is equivalent to that of more than 50 Applied Biosystem's 3730XL capillary sequencers and at about one-sixth of the cost. Since first introduced to the market in 2005, next-generation sequencing technologies have had a tremendous impact on genomic research. With the publication of more than 100 research articles in less than two years, NGS has demonstrated its enormous potential on metagenomics, transcriptomcs, resequencing of previously published reference bacteria strains, discovery of new virus, microRNA/small RNA profiling and population genomics. We are using the Solexa and SOLiD sequencing platforms to perform small RNA profiling and RNA-Seq. We also developed a series of bioinformatics packages to analyze the datasets generated. |
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| ¡@ | Wikipedia on DNA sequencing |
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| Proteomics | |
| ¡@ | Proteomics is one of the major research in the Chang University. The "Proteomics Core Laboratory" is equipped with nine Mass Spectrometer. We routinely use 2-dimenional gel electrophoresis/MALDI/TOF and LC/MS/MS to profile and identify protein expression levels. |
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| ¡@ | Chang Gung Proteomics Core Laboratory |
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| microRNA | |
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MicroRNAs (miRNAs) are ~22-nucleotide non-coding RNA species that are found in diverse organisms from viruses, plants to mammals. MicroRNAs are encoded by specific genes and play key roles in regulating the translation and degradation of mRNAs by inducing post-transcriptional silencing through base pairing to complementary target sites. We identified the first miRNA in T. vaginalis by direct cloning and stem-loop real-time-PCR. Up-to-date, miRNA have only been reported in Giardia lamblia and T. vaginalis experimentally. |
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| ¡@ | Wikipedia on microRNA |
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